- Title
- An amperometric biosensor based on glucose oxidase immobilized in a sol-gel polyvinyl alcohol/silica hybrid composite film on a prussian blue modified electrode
- Creator
- Zuo, Shao-Hua; Zhao, Hong-Li; Zhang, Ling-Fan; Yuan, Hui-Hui; Lan, Min-Bo; Lawrance, Geoffrey A.; Wei, Gang
- Relation
- Advanced Science Letters Vol. 3, Issue 4, p. 476-481
- Relation
- http://www.aspbs.com/science/contents-science2010.htm#v3n4
- Publisher
- American Scientific Publishers
- Resource Type
- journal article
- Date
- 2010
- Description
- An amperometric glucose biosensor was fabricated by entrapment of glucose oxidase in a composite matrix of silica sol-gel and polyvinyl alcohol physically layered on top of a Prussian blue-modified Pt electrode. This biosensor functions through the detection of hydrogen peroxide produced by the glucose oxidase-catalyzed oxidation of glucose at -0.05 V. The biosensor exhibited high selectivity, and a sensitivity of 3.41 μA/mM, due to a combination of electroreduction of H₂O₂ at the surface of the modified Pt electrode at low potential and a sympathetic microenvironment for enzyme immobilization provided by the silica sol-gel. The biosensor had a linear response to glucose over the range 5.0×10⁻⁵ to 2.5×10⁻³ M, with a response time of less than 8 sec. The detection limit of the biosensor is 4.0 μM, based on a signal-to-noise ratio of 3. The biosensor exhibits satisfactory long-term stability; 85% of its original activity was retained after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4 °C.
- Subject
- amperometric glucose biosensor; biosensors; prussian blue-modified electrode; enzyme-based glucose
- Identifier
- http://hdl.handle.net/1959.13/932565
- Identifier
- uon:11386
- Identifier
- ISSN:1936-6612
- Language
- eng
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